ATP Production By Beer Yeast Cells Immobilized In Open Pore Gelatin Matrix

Using immobilized yeast cells to yield adenosine tripHospHate (ATP) from adenosine monopHospHate (AMP) is one of the most interesting techniques in ATP industry. The key techniques in the ATP production are the steady of the cell quality, the appropriate method of immobilization and operational conditions. In the dissertation, the cultivation of yeast, the preparation of immobilized cells in open pore gelatin batrix and the operational conditions of ATP fermentation were investigated. Besides, the separation and purification technique of ATP production was built, the ATP recovery, the purity of ATP product and virtual productivity were also discussed with the experiment.First of all, a strain of beer yeast which has high conversion rate but low stability was selected. According to orthogonal experimental design, the effects of four factors (contents of cane sugar honey, peptone, (NH₄)SO₄ and KH₂PO₄) at three levels on the cells concentration and the ATP conversion activity were investigated and then the optimal ratio of components in the expanding culture medium were determined. The results are: m(cane sugar honey)=12%, m(peptone)=0.70%, m ((NH₄)₂SO₄)=0.25% and m(KH₂PO₄)=0.15%. Then, it was found that the appropriate expanding culture time was 14～20 hours.Secondly, it was selected to study the preparation of immobilized cells in...