Application Of Microfluidic Chips For Pharmaceutical Analysis

In recently years, the chip-based capillary electrophoresis (CCE) has achieved great progress and becomes essential technique of the miniaturized total chemical analysis system(μTAS or Lab-On-a-chip). Thanks to the advantages such as low sample consume, quick analysis speed and easy to be miniaturized, CCE possesses promoting potential in analysis and the life science. In this text, two methods have been developed for the determinations of ephedrine dopes and of the anti-tumor medicine by means of a chip-based capillary electrophoresis system with laser-induced fluorescence detector(CCE-LIF).In the first chapter, the device and the characteristics of the CCE-LIF, the reagents and the methods of derivatization reaction were introduced,and the reports on application of CCE-LIF for pharmaceutical analysis were reviewed.In the second chapter, a method was developed for determination of ephedrine (EP) and pseudoephedrine (PEP) dopes with a CE-LIF. The dopes were derived with 7-chloro-4nitrobenzo-2-oax-1,3-diazole (NBD-C1) to form fluorescent products. EP and PEP can be base-line separated from the reagent components as well as the species existing in the urine within 90 seconds. There are good precision and linear relationship for both EP and EPE. Satisfactory recoveries were achieved when the developed method was used for determination of EP and PEP concentration spiked into human urine.In the third chapter, a method was developed for determination of anti-tumor medicine doxorubicin (DX) and daunorubicin (DAU) with a CCE-LIF. Addition of 40% acetonitrile into the borate running buffer could significantly improved the separation efficiency and enhanced the fluorescence detection sensitivity, resulting a base-line separation of the two drugs within 60 seconds. Satisfactory repeatability and the limits of detection(LOD) were achieved.