| Nowadays, as an important part of μTAS microfluidic chip is very prevalent in the development of analytical chemistry. The most important character in the structure of microfluidic chip is all kinds of micro-scale channels. The analytical capability of microchips is achieved by the control of fluidic channels. So the research of micropumps that adjust to micro-scale channel is the basis of achieving microfuidic handling. Over the last decade, electroosmosis has been used extensively in the microfludic transport. This simple method is very advantageous in moving a fluid freely even in complicated microflow channels connected with each other. But the high drive voltage and the power source much larger than microchip itself present great problems. Another possibility to achieve controlled fluid handling is to use pumps and valves. This conventional approach is considered to be a good handling method in the coming years. However, many of them are difficult to fabricate and the integration into a microsystem do not seem to be totally successful at present.Based on the literatures about the study of polydimethylsiloxane peristaltic micropumps and chemiluminescence detection in μTAS for these years, this thesis brought forward a novel approach for microfabrication of modified pneumatic micropump on microfluidic chips with which the double fluid channels were controlled synchronously. The performance of the system was tested using K3[Fe(CN)6] as a model sample with chemiluminescence detection by the luminol-K3[Fe(CN)6]-H2O2 reaction. The results obtained indicates with the device K3[Fe(CN)6] can be detected at a concentration of 8×10-7mol/L and with a RSD of 0.9%(4×10-5 mol/L, n=7), a linear calibration was obtained from 6×10-6 to 6×10-3 mol/L. The linear regression coefficient R2 is 0.9990.Due to the pneumatic micropumps on glass-PDMS microfluidic chip the difficulty of process and cost of stuff were reduced, and it's rigidity intension was greatly enhanced at the same time. Furthermore, the successful handling of multiple channels made the stability and integration of the microchip improved.
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