Studies On Technology Extraction And Crystallization Puerarin Process Of Supercritical Fluid

With the research and development of natural products, the active compounds in which, especially flavonoids have been the focus of drug materials R&D. Pueraria is a kind of active resources for drug and food since it can provide people with a lot of nutriments and particular medicative effects. It has many uses . As the main active component of pueraria, in which puerarin is paid more and more attention increasingly for its good therapeutic effects to cardiovascular diseases. The separating methods of puerarin appearing in too many documents are mainly distilling/extracting, absorbing by maroreticular resin, solvent crystallizing, all of these methods are mostly some defects such as environment pollution, more process, long time, and so on.Aiming at puerarin coexisting in a complicated mixture with protein, sugar, isoflavone, and so on. This dissertation chose puerarin as an object to high effective separation with supercritical fluid extraction and crystallization technique, the author could achieve separation between target product and impurity basically, and building new separation technology of puerarin with high efficiency, celerity and no solvent pollution.In our experiment conditions, the author had obtained the following results: the process of supercritical CO₂ extraction and crystallization can purify puerarin from purity of about 40% to 80% or so, and crystallization ratio was about 45%, the optimal parameters of supercritical CO₂ extraction and crystallization were as follow: material dosage 50g, pressure 17MPa, temperature 55℃ and time 90min;The whole processing is simple and efficient,the whole process can greatly enhance produce efficiency and reduce technics steps.