Study Of Alcohol Fermentation By Raw Starch

Now, the requirement amount of the alcohol is becoming larger and larger. At the same time, considering the environment protect and energy saving, the method of alcohol fermentation must be reformed. Recently, as the development of bioscience, a lot of enzymes were used in the alcohol fermentation process. The most used fermentation method includes liquefaction, saccharification and fermentation (two enzyme method). Recently, a new to product ethanol by simultaneous saccharification and fermentation (SSF) method was reported by American researchers, the SSF method simplifies the alcohol fermentation process by combining the saccharification and fermentation into a single step. The saccharification and fermentation are the most important processes in alcohol fermentation by using raw starch. We study the raw starch saccharification firstly and then study the sugar (glucose) fermentation. In raw starch saccharification experiments, inhibition phenomena and enzyme inactivation were studied. In alcohol fermentation by glucose experiment, new alcohol fermentation kinetic models were setup and the new models can fit the experimental data well. Finally, raw cassava starch fermentation with SSF method was experimented. The experimental results show that the SSF method spends shorter time and produces more amount of alcohol comparing with the "two enzyme method". This means that the SSF method is superior to the "two enzyme method ".