Studies On The Difference Expression Of The Enzymes And Proteins Of The Fish Liver Mitochondria Acted By Hexachlorobenzene And Cadmium(Ⅱ)

Studies on Environmental Toxicology recently focus on the toxic function and mechanism of persistent bioaccumulative toxic pollutants (PBT) on organism. The structure and function of mitochondria are extraordinary; they are the main targets of the contaminant toxic function. In this thesis, the toxic function and mechanism of Hexachlorobenzene (HCB) and Cadmium (â…¡) with mitochondria islated from Carassius auratus liver were studied. The main contents are summed up as follows:(1) The effects of HCB on the main antioxidant enzymes (SOD and GSH-Px) and malondialdehyde(MDA) in mitochondria were studied. The results show that the activity of SOD and GSH-Px and the quantities of isozymes increased at the low concentration of HCB, while they were restrained at the high concentration of HCB. The gsh3 of GSH-Px was restrained completely at the high concentration of HCB. The concentration of MDA in mitochondria changed inconspicuously at the low concentration of HCB, while it increased conspicuously at the high concentration of HCB.(2) The effects of HCB on main energy metabolism enzymes(COX,Ca~2+-ATPase) in mitochondria were studied. The results show that the isozymes of COX were activated with the low concentration of HCB, while they were restrained at the high concentration of HCB. The C0X1 was restrained completely at the high concentration of HCB. As the concentration of HCB increasing, the quantities of isozymes in Ca~2+-ATPase decreased and were restrained completely in the end. The Ca~2+-ATPase2 and the Ca~2+-ATPase3 were restrained completely at high concentration of HCB.(3) The effects of HCB on the protein expression in mitochondria were studied. The results show that the quantities of protein bands increased firstly and then decreased at the concentration of HCB increasing. The polypeptide (a) was expressed at the low concentration of HCB and the expression quantities of polypeptide (b) decreased and disappeared in the end with the concentration of HCB increasing.(4) The effects of Cd~2+ on the main antioxidant enzymes (SOD and GSH-Px) and malondialdehyde(MDA) in mitochondria were studied. The results show that the activity of SOD and GSH-Px and the quantities of isozymes decreased and were restrained obviously with the concentration of Cd~2+ increasing. The gsh3 of GSH-Px was increased with the concentration of Cd~2+ increasing.(5) The effects of Cd~2+ on main energy metabolism enzymes(COX, Ca~2+-ATPase) inmitochondria were studied. The results show that the expression quantities of COX changed inconspicuously at the low concentration of Cd2+, while at the high concentration of Cd2+ they were restrained, the cox3 and the cox5 appeared. The quantities of isozymes in Ca2+-ATPase decreased with the concentration of Cd2+ increasing.(6) The effects of Cd2+ on the protein expression in mitochondria were studied. The results show that the expression quantities of the protein, of which the molecular weight (MW) exceeds 66.2kD decreased with the concentration of Cd2+ increasing, while the expression quantities of the protein, of which the molecular weight (MW) is under 66.2kD increased firstly and then decreased remarkably with the concentration of Cd2+ increasing.