Studies On Process For Isolation And Purification And On Method Of Quality Control Of Effective Constituents From Hypericum Perforatum L.

Hypericum perforatum extracts and their preations are the most popular anti-depression herbal drugs in north America and Europe. It is effective for the treatment of mild to moderate depression,which has been verified by numerous preclinical and clinical studies. Fewer side effects has been detected compared with traditional antidepressants. It is showed by newly study that its active principle More and more pharmacology effects has been discovered lately ,such as anti-virus, anti -cancer , hepatipis healing, leucocythemia healing. Two kind of the active principle,hypericin and flavone, was studied by quantitative and quanlitive analysis,extraction technics, isolation methods, purification methods, and chromatogram methods aiming to develop and take best advantage of hypericum perforatum. Its isolation and purification and extration methods was obtaine. The ralated quatitative and qualitive analysis was established. Two products which rich in hypericin and flavone repectively were prepared through simple and stable preparation precess. The content of hypericin is higher than 40%(UV) in hypericin rich product. The content of flavone is higher 80% (UV) in flavone rich product.The content of hypericin is as much as 89% in the product which is prepared by HPLC. The main works and conclusions are included as follows: (1) Active constituents extraction in hypericum perforatum The extraction process of hypericin and flavone was studie by one dimension investigation. The effects of dosage of solvent, time and temperature of extract were investigated in detail, resulting in an optimum condition to extract hypericin and flavone. The results are: 35 times of 80% ethanol solution, 1.5h ultrasonic extraction under 40℃. The extraction rate of hypericin and flavone are 93%, 88% respectively. (2) Isolation and purification in hypericum perforatum The big pore resin was utilized to isolate and purify hypericin and flavone in hypericum perforatum.HPD-100 resin was selected to isolatate hypericin and flavone because it has larger adsorption and better desorption.40% and 60% ethanol solution were adopted to wash off hypericin and flavone in the mode of grads. Both hypericin and flavone were obtained as desorption solution. 40% hypericin and 80% flavone was got after these two desorption solutions were purified further by HPD-100 and D-101. (3) HPLC analysis and preparation of hypericin. The quantitative analysis method was established as the following: Flowing phase, methanol-ethyl acetate-0.1M NaH2PO4(514:208:278 ); Velocity of flow, 1.0Ml/min; The inspected wavelength, 590nm; Temperature of chromatogram columniation, room temperature. The HPLC preparation method was concluded on the base of the analysis method. The chromatogram condition of preparation shows : Flowing phase, methanol-ethyl acetate -0.1M NaH2PO4(615:235:150); Velocity of flow, 3ml/min, 2ml injection at once; The inspected wavelength, 590nm; The product rich in 89% hypericin was prepared and can be used as the control sample.