Research Of Paclobutrazol And Nicotinamide Molecularly Imprinted Polymers

Molecular imprinting technique (MIT) is a polymerizing technique to produce molecular recognition materials with high selectivity and affinity. Polymerization is occurred between functional monomers and crosslinker around a template. Extration of the template forms polymer with molecular memory to the template. It has many applications on the fields of analytical chemistry, catalysis and organic synthesis. In this paper, four chapters were consisted. In Chapter I: The principle of molecular imprinted polymers (MIPs), polymerization methods, application of this technique and its future developments were introduced. In Chapter II: A synthetic polymer selector for nicotinamide is prepared by molecular imprinting technology. Chromatographic analysis showed that the nicotinamide-imprinted polymer is capable of recognizing the functional difference of the two analogues in aqueous phase. Furthermore, the adsorption isotherms of nicotinamide were determined using static method. The binding characteristics of the nicotinamide on the imprinting polymer were evaluated by Scatchard analysis. In Chapter III: Paclobutrazol has been employed as template for the preparation of in-situ molecularly imprinted polymer and the continuous rod has been applied as liquid chromatographic stationary phases for the separation of paclobutrazol and its analogies. The characteristics of adsorption and thermodynamics of paclobutrazol -MIP were investigated. The adsorption isotherms of paclobutrazol were studied by static method. Schatchard analysis indicated that two classes of binding site were present in the imprinted polymers. The results showed that the MIP had significant molecular recognition ability and it had high affinity and selectivity for paclobutrazol in aqueous system, by which molecularly imprinted solid phase extraction (MISPE) was studied for trace analysis of paclobutrazol in water. In Chapter Ⅳ: A method for the determination of folic acid and related compounds with high performance liquid chromatography has been established. Folic acid and its related compounds were successfully separated on a JASCO C18 column (250×4.6mm i.d.,5 μm)...