Circumstance Control On Processing Of Tea And Analysis On Its Biochemical Qualities

Two aspects were studied in this article: First, in order to analyze material characteristics, we distilled polyphenol oxidase (PPO) separately from Fuyun No. 6, Maoxie, Tieguanyin, Huangdan, Rougui; and examined properties of these enzyme and their influence factors. Later, the effects of different parameters and controlled atmosphere drying(CAD) on the quality of green tea were analyzed. Mathematical model was also established. Infusing properties of Green tea dried with hot air and CAD were compared. The results were as belows:In the reaction system of 1.0 ml PPO, 1.0 ml 0.1 mol/1 catechol substrate and 3.0 ml 0.01 mol/1 PB alleviant, the activity of PPO from each different frcash increased curvely with the increase of catechol substrate volume when catechol was in a certain concentration; enzymatic activity from Fuyun N0.6 was the highest when the ratio of the catechol substrate content to PPO content was 2:1; While the activity of PPO from Maoxie, Tieguanyin, Huangdan, Rougui was the highest when the ratio of the catechol substrate content to PPO content was 1.5:1.The most suitable temperature for Fuyun No.6 PPO was (35~40)°C; the activity of PPO from Maoxie, Tieguanyin, Huangdan, Rougui changed with " within (45~65)°C, and their temperature peaks appeared at 45°C ,55°C, 65°C.Under the condition of (50-90) °C hot water treating for 1h, the PPO activity all lost in some degree. The PPO could act at alkaline conditions, the best enzymatic activity was detected at pH 8.6. All the five materials tested showed some degree of...