Study On The Role Of G6PD In The Elicitor-mediated Taxol Biosynthesis And The Mechanism Of Its Antioxidation

Producing taxol from Taxus suspension cultures is always the cynosure of plant naturalproducts research. Treatment of Taxus Chinensis cell suspension cultures with fungalelicitor is regarded as one of the most efficient ways to enhancing taxol production.Previous research shows while boosting taxol production by fungal elicitor treated TaxusChinensis cell suspension cultures, it is observed that there is concomitant change inglucose-6-phosphate dehydrogenase(G6PD). However, the roles of G6PD in taxolproduction remain unknown, which is the main subject of the present dissertation. In thisarticle, we also discuss the functionary mechanics of G6PD antioxidation inelicitor-mediated taxol production. Our results are listed as follow: 1,G6PD, similar with other enzymes in plant defense reaction against diseases such asperoxidase(POX), polyphenol oxidase(PPO) and so on, involves in plant defense strategy. Itis speculated that G6PD plays important roles in plant defense strategy by providing C5compounds, like that phenolics compounds, then increases the resistance to pathogen attack,and providing the more NADPH, which can relieve the oxygen stress in hypersensitivereaction. 2,Our results indicated that G6PD played a critic role of Taxol production by affectingdirectly and indirectly cell viability. G6PD also play antioxidation role while elicitortreatment leads to increasing taxol production. 3 , The facts that glutathione content do not decrease while inhibiting malicenzyme(ME) activity shows that ME is not the main resource of NADPH as potentialregulation to antioxidation in Taxus chinensis cells. G6PD is the important resource ofNADPH in Taxus chinensis cells. The antioxidation capacity obviously decreases whileinhibiting G6PD activity. 4,It is concluded that the role of G6PD in antioxidation in Taxus chinensis cells isplayed by transfer electron from NADPH to reduced glutathione, which is the importantcellular antioxidant.