Studies On The Phospholipid Of Rape Oil Residue

Phospholipid were first found from humanbeing brain by Uauquelin in 1812 and soyabean phospholipid were found in 1930.1n recent years, phospholipid are got excessive attention . The research indicates that phospholipid have the function of activating ceil, promoting brain growth, improving artery blood vessel structure, keeping body water and participating in body metabolize etc .Phospholipid of rapeseed oil was in-depth observed to it's chemical decolor and aikylate modify.At first, acetone was used to extract and separate phospholipid of rapeseed oil residue, which was discolored by hydrogen peroxide ,We obtained relevant kinetics parameter of discolored reaction, and the test results as follows:(1) Obtained the optimal technical parameter of discolored :reaction temperature is 65 ℃,hydrogen peroxide concentration is 4% and discolored time is 30 minute.(2) Obtained the relevant kinetics parameter of discolor : reaction order of hydrogen peroxide concentration to decolorizing effect is 0.6199, chromopexis concentration to decolorizing effect is 0.3329 and activation energy is 52.88Kj/mol.(3) decolorizing influence to phospholipid content: we can think that using hydrogen peroxide for decolor to phospholipid almost didn't effect phospholipid content , it also meaned that phospholipid loss was very small.(4) decolorizing influence to phospholipid quality : after decolored , iodine value of phospholipid was reduce ,which indicated decolored degree better ; emulsificed stability of phospholipid enhance , which meaned emulsification increase ; phospholipid chroma deduced 40% that indicated decolorizing effect relatively ideal ; decolorizing process made peroxide value increase , which was not anticipant.As a whole , making use of hydrogen peroxide as a bleaching agent to decolorizing phospholipids of rapeseed oil is a very effective method , so it was value to popularize.Then , putting excessive bromethane to phospholipid of rape oil for aikylate modify and using HPLC method to detect content of not modified phospholipid and modified phosphlipid , then weobtained the result as follows: .(1) Obtained lecithin appear apexes time: HPLC was established to detect appear apexes time of lecithin contrast, which was 6.267 minutes ,so we can get lecithin apexes time of sample.(2)Obtained alkylate reaction technical parameter :reaction time of alkylate of rapeseed oil phospholipid was 2 hours .after 2 hours the reaction almost cease; this reaction by temperature influence was small ,which can be processed at room temperature ,yet once temperature excess 40℃, modified agent may volatilization.(3) During alkylate reaction , we obtained lecithin content of sample is 1.43 times to of before modified , and we also get from HPLC chromatogram chart that there were byproduct come into being,so we can't assure cephalin whether or not deduce.At last, alkylate modified of rapeseed oil phospholipid whether of not succeed need more testified.