| The simultaneous determination of components in multicomponent drugs is always encountered in pharmaceutical analysis. Paracetamol, Caffeine and Chlorphenamine maleate are main compositions of medicine for the cold. This paper tells the determination of each composition in the write system consists of above drugs by chemometrics and UV spectrophotometry. UV spectrophotometry combined with chemometrics to determinate multicomponent drugs is very valuable for its accuracy, simplicity and low cost. The chemometrics methods dealt with in this article are multiple linear regression (MLR), K metric method (K) and P metric method (P). Comparing the results of each method, conclusion can be made as following: MLR, K and P are all suitable in this system, however, when multi-affect exist, indirect calibration (K, P) is better than direct calibration (MLR). And P is the best of all in this system, the mean recovery percentage and RSD of Paracetamol, Caffeine and Chlorphenamine maleate are as following, 96.12%,1.41%; 98.30%,1.30%;101.42;1.08%. Condition such as solvent and wavelength are also discussed, best condition was get. As parts of interaction research, this article also use UV Spectroscopy to investigate the inclusion of β-cyclodextrin (β-CD) with paracetamol and the interaction of chlorphenamine maleate with bovine serm albumin (BSA). Method of analysis interaction by UV spectroscopy is founded when spectra overlays. Therefor broaden the area of UV spectroscopy in interaction research. β-CD interact with paracetamol and form inclusion compound in water. The inclusion constant is 4.36×102 l/mol. The interaction of Chlorphenamine maleate with BSA belong to electrostatic model, they are combined by electrostatic attraction. According to this model, the constant is 1.32×105 l/mol, The max mol ratio is 40. The concentration of Chlorphenamine maleate has strong effect to the reaction, time has few effects, and from 5℃ to 22℃, Constant has few changes. |
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