The Biochemical Treatment Of Leaking Oil-containing Medium In The Industrial Recirculating Water

In the industrial recirculating water treatment, it is a new subject to treat leaking oil-containing medium with biological technique. By the process of domestication, the petroleum degrading strains N-1 and N-2 which are isolated, screened and purified from oil-containing sludge can grow up and breed under the condition of T=39癈, pH=9.0 and facultative anaerobic culture. They are adapted radically to the growth environment of recirculating water system and have certain degradation capability for leaking oil-containing medium in the system. Through morphological observation and a series of physiological and biochemical character experiments, N-l and N-2 are appraised to be Arthrobacter sp. and Pseudomonas sp. respectively.The experiment results show that when the strain suspension which is inoculated into 100ml sample is 5ml and the degradation time is 5 days, the degradation rate reaches the maximum value and then approches to be steady. The maximum degradation rate of N-1 is 75.3% and 68.2% for 15* lubricating oil and crude oil respectively. Corresponding the maximum degradation rate of N-2 is 82.5% and 77.9% respectively. The degradation rate increases first and then decreases with the increase of inoculated strain quantity. In the range of lower concentration (< 500mg-L-1), the degradation capability of strains increases with the increase of oil concentration. In the range of higher concentration ( > 500mg-L-1), the degradation capability of strains does not change with the change of oil concentration. At the same time, there is no obvious mutual influence between N-l or N-2 and the corrosion inhibitor in the recirculating water system. And the oxidizing type bactericide or non-oxidizing type bactercide has a better bactericidal effect for N~1 and N~2.