A Study On The New Methods For The Determination Of Proteins And Pharmaceuticals By Resonance Rayleigh Scattering

Resonance Rayleigh scattering(RRS) method is a new quantitative analytical technique which has progressively developed since 1990s . In recent years, RRS method has brough to more and more attentions because of its simple operation, high sensitivity and fairly good selectivity . It has been mainly applied in biological macromolecules and inorganic ions. In certain conditions, proteins exist as a big positive ion It can react with anion which has counter charges and then leads to the significant enhancement of RRS as well as the appearance of new RRS spectra. In view of this, we found out three new system that could be used in proteins determination, and studied the RRS spectal characteristics, the affecting factors, the properties of analytical chemisty and their analytical applications. Pharmaceutical analysis is an important content of analytical chemistry, but so far there is a little studies which use RRS method . In certain conditions, some medicines exist as big cation or anion, and some dyes coexist as big ion with counter charge . They can combine each other and then result in the significant enhancement of RRS and the appearance of new spectra of RRS. Based on this, we researched the interac~tion of two new medicines which named Raloxifene and Sildenafil with two kind of acidic bisazo dyes and found out some new systems that could be used to determine those medicines . The RRS spetral characteristics, the primary influent factors, the properties of analytical chemistry and the analytical application of every system have been studied too . Thus we set up some new simple, quick and sensitive methods to determine the two medicines by RRS technique. 1. Some Bisazo Dyes of Chromotropic Acid-Proteins Systems In sodium citric acid-HC1 buffer solution (pH3.4-4.O), some bisazo dyes of chromatropic acid such as Arsenazo M(AAM), Chloi~ophosphonazo IJ1(CPAIIL) and Chlorosulphonphenol S(CSPS) combine with proteins, causing enhanced RRS in the range of 400?70nm and the maximum RRS peak at 470nm . Their calibration graphs are linear over the range of O?.6pg mU?for CPAIII-BSA system, O?.8pg mU?for AAM-BSA system and O--4.8~ig mU?for CSPS-BSA system separately The method has high sensitivity with the detection limits of BSA (a 3) is I 8.5ng mL~?CPAJll), 21 .7ng mL?CSPS) and 27.9ng mL?AAM), and selectivity is fairly good with the investigation for the influence of some coexisting substances . This method can be applied to the determination of water-soluble proteins in the tea. 2. Some Bisazo Dyes of Chromotropic Acid-Th( LV) Chelate with Proteins Systems In pH 1.4-1.9 acidic solution(sodium citrate-hydrochloride buffer), some bisazo dyes of chromotropic acid such as Arsenazo III (AA 111), Arsenazo M(AAM), Chlorophosphonazo llI(CPAIII) and Chlorosulphonphenol S(CSPS) can react with Th(lV) to form the corresponding chelate anion, and then further interact with some proteins to produce a ternary complex, causing significantly enhanced RRS in the range of 400-470nm and the maximum RRS peak at 470nm . Their calibration graphs are linear over the range of 0 .6pg mU?for Th( IV )-CPA In system, 0?.8pgImL~?for Th(LV)-AAM system, 0?.Opg mU?for Th(LV)-AAIU system and 00.28pg mU?for Th( LV )-CSPS system separately . The method has high sensitivity, and the detection limits of BSA( a =3)is 1O.7ng mL?Th(LV)-CPAIII), 6.3ng mL?Th(IV)-CSPS), 13.6ng mU?(Th(IV)-AAIII) and 22.ing mU?Th(IV) - AAM)respectively, all of the...