| Neural stem cells are defined as cells which possess the capacity about self-renewing and multi-directional differentiation potentiality like neurons, astrocytesin and oligodendrocytes which exist in various regions of the central nervous system throughout the mammalian lifespan. The present study was initiated to observed the growth characteristic and the features of serum induced differentiation of hfNSCs, and search a high-performance method that can be used for procuring and propagating NSCs in vitro. To explored a feasibility source of suitable cell for NSCs-based therapy.Brain tissue of aborted human fetal aged 8-16 weeks gestation, Neural stem cells were cultured in serum-free growth medium, the proliferation capability of the cells was observed. Brdu incorporation assay the percentage of Proliferation was about 37.9% and the Cloning efficiency was about 6%-7%. Neural stem cells were induced to differentiate in DMEM/F12 with 10% fetal bovine serum but without the growth factors. Anti-Nestin, anti-MBP, anti-Tuj-1 and anti-GFAP were used in Immunofluorescence labeling. Tuj-1-positive cells were smaller and had a clear fringe. The proportion of Tuj-1-positive in differentiated NSCs from striatum was about 56.8%. The GFAP-positive cells were biger than the other kinds cells, and the appearance of them was irregular. The proportion of GFAP-positive in differentiated NSCs from striatum was about 43.2%. The MBP-positive cell was never seen in the differentiated NSCs from striatum in our study. The nestion-positive cells were located interior the neurospheres. The Tuj-1-positive cells and GFAP-positive cells were all nestion-positive but the fluorescence of differentiated was dimmish compared with the cells before differentiation.Neural stem cells can be harvested from human fetal brain, and the cells maintain their undifferentiated features and have the capacity of self-renewal and multi-directional differentiation potentiality in vitro.
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