Study On The Culture In Vitro And Proliferation And Differatiation Of Different Gestatational Age Human Embryonic Neural Stem Cells

PART 1 ISOLATION, CULTIVATION, PURIFCATION AND IDENTIFICATION OF DIFFERENT GESTATIONAL AGE HUMAN EMBRYONIC NEURAL STEM CELLSBackground and Objective: Recently, neural stem cell (NSC) is one of the hot spots in neuroscience. The study showed that there was spacious clinical application perspective in transplanting NSC to acute or chronic nervous system injury, such as multiple sclerosis (MS), Alzheimer's disease (AZ), Parkinson's disease (PD), hereditary chorea, ischemic encephalopathy and so on.Not only the preclinical study at the present stage but also clinical application in the future, large numbers of NSCs is needed. So it is important to isolate, cultivate, and differentiate NSC. To establish stable and reliable methods of culture in vitro of NSC is foundation to explore its mechanism of proliferation, differentiation, and transplantation. The aim of this study is to obtain different gestation age human embryonic NSC, and...