MiR-34c Inhibits Glioma Growth And Invasion Through Repressing Raf1

It has been shown mir-34c play an important role in tumorigenesis through modulating the p53 initiated gene expression and to serve as a tumor suppressor in various tumors. Here, we report that mir-34c is down-regulated in glioma and the DNA methylation state of 18 CpG inucleotides upstream of the miR-34c gene causes it down-regulation. Induction of miR-34c leads to Gl arrest and inhibits glioma growth and invasion. Moreover, Raf-1 has been identified as a novel target of mir-34c. Mir-34c transfection could repress the Raf-1 expression. Our data indicate that mir-34c might be a novel tumor suppressor in glioma and a potential therapeutic target in brain cancer therapy.