Optimization Of The Biosynthesis Conditions Of Marine Bacteriocin 1701

Screening of novelty microbial medicine from terristrial mircoorganisms has become increasingly difficult, while the marine mircoorganisms have a great potential in produing a variety of novelty and unique bioactive substances. According to previous studies in our laboratory, the marine strain 1701 may have a potential value for further developing and utilizing. It has become very necessary to optimize the biosynthesis of marine strain 1701, since the concentration of marine active secondary metabolites in fermentation broth is very low, and for the purpose of having sufficient amount of antibacterial activity of pure ingredients prepared for the further studying. The main results of this paper are summarized as follows:(1) Application of single-factor method for screening the best nutritional and cultural condition for the biosynthesis of marine strain 1701. The best carbon source is glycerol, organic nitrogen source is fresh fish peptone, inorganic nitrogen source is NH4Cl, mineral salt is NaCl and MgSO4, the best incubation temperature at 28℃, the initial pH at 8.0.(2) Screened by Plackett-Burman design, the significant factors which affect the biosynthesis of marine strain 1701 are the concentration of fish peptone, NaCl and NH4Cl.(3) Through the RSM, the level of biosyntheis of marine strain 1701 raised 1.4 times in the shake flask.(4) In 5L fermentor, Found that the highest level of biosynthesis of marine strain 1701 appeared when the level of pO2 was set at 30%, according to the different levels of pO2 under the linkage model.(5) Training the data of the first 4 batches of fermentation by the ANN, the mapping relationship between input (t, Temp, Turb, and pH) and output (ΔDe) has been set up, and the call format of trained ANN can be wrote as: a=sim(net, p).(6) Using GA to evaluate the biological genetic evolution, and finally found out the optima controlling curve of the fermentation. Eventually, the level of biosynthesis of marine strain 1701 increases by about 1.4 times under the control of fermentaion curve.