Phylogenetic Diversity Of Culturable Bacteria Of Two Sample Site In The Western Kunlun Mountain, Northwestern China

To date.Previous studies have recovered culturable bacteria from alpine cold envirment around the globe and in our country.However, description of alpine soil microbial communities are limited and their existence in the Kunlun mountain have not been previously explored.This study is first attempt to describe the phylogenetic diversity of a culturable bateria communites from alpine soil in the westren Kunlun mountain in the northwestern China.Soil samples of two different see level sites were used.Bacterial isolation was conducted on plates of LB and TSA,followed by 16S rRNA indentification and phylogenetic analysis.The statictic analysis was carried on by results of the 16S rRNA indentification and phylogenetic analysis to the all bacterial community and in the two different soil with different plates.In total 91 bacteria were obtained, belong to 25 genus and 51 species.Analysis of 16S rRNA sequence and phylogenetic tree showed that the bacterial communities belong to three major groups:the low G+C Gram-positives,the high G+C Gram-positives,γ-proteobacteria.The most abundant and diverse isolation were members of Gram-positive bacteria,particularly the low G+C Gram-positives as a dominant group in the alpine soil culturable populations;with 27 species and 10 possible novel species including,Bacillus,Brevibacterium,Virgibacillus,Oceanobacillus ,Gracilibacillus ,Paenibacillus ,Desemzia ,Sporosarcina ,Planomicrobium ,Planococcus ,Staphylococcus ,Jeotgalicoc-cus,Exiguobacterium.The high G+C Gram-positives were the second subgroup of high occurrence, among which Arthrobacter is relatively abundant. The biological diversity index displayed that Simpson's diversity index D was 0.9245, Shannon-Wiener index H was 2.6080 and Shannon-Wiener's evenness index E was 0.5186 and indicated the better microbial diversity in the habitat.Generally ,the 3100m sea level soil had more isolates of low G+C Gram-positives andγ-proteobacteria than 2795m sea level soil,but less high G+C Gram-positives.In addition, the biological diversity index displayed that the 3100m sea level soil had higher diversity index than 2795m sea level soil. The dominance index of two medium showed that the isolates of TSA medium had higher bacterial diversity. The isolates of low G+C Gram-positives and high G+C Gram-positives are rich in western Kunlun mountain,particularly the low G+C Gram-positives as a dominant group in the alpine soil culturable populations,which deserve further study and exploitation.