Vertical Distritubion Of Archaeal Diversity In Surface Sediment From The Western Pacific

Due to the special environment characteristics of the deep-sea such as cold, high pressure, dark and poor organic carbon, the biosphere in the deep-sea environments was markedly different from the continent and the shallow sea. In addition, because of the rich abundance and special metabolism pathways of microorganisms inhabiting in the deep-sea environments, the deep-sea has been considered as an important reservoir of biological resources for exploration.The sediment samples used in this study were collected from the MD06-3057 site in the West Pacific Ocean (12°36'39"N,104°19'28"W).The stations located in the central Philippines, sibuyan sea, depth of 1666 meters. The core was 9.6 meters long, each 0.5m as a research scale.Diversity of archaeal was studied in deep-sea sediments from the Western Pacific by PCR-RFLP and sequence analysis of 16S rDNA and comparing with the published sequences in GenBank data-base. Archaeal 16S rDNA sequences were within phylums of Crenarchaeota and Euryarchaeota, respectively. The majority of archaeal phylotypes were Marine Benthic Group B (MBGB), Marine Benthic Group D (MBGD) and Marine Crenarchaeotic Group (MCG).Additional sequences grouped with the South African Gold Mine Euryarchaeotic Group (SAGMEG),Deep-Sea Hydrothermal Vent Euryarchaeotic Group (DHVEG),UCIIB,VALIII and Marine Benthic Group E (MBGE). These results indicate that archaea are abundant and diversified in surface environment of subseafloor sediments.