Response Of AtCBP60g Under Biotic And Abiotic Stresses

Calcium is known as a second messenger involved in plant growth, development, stress resistance and other physiological responses. And calmodulin as a calcium channel relay sensor, mainly by binding to CaM-binding protein (CaMBP) to regulate the cell physiological and biochemical reactions, plays a crucial role in Ca²⁺ signaling system. In Arabidopsis, at least 200 CaMBPs are ecoded in the genome. Five members in a small family, CBP60s, were studied by Reddy et al in 2002. But the other two members, At4g31000 and At5g26920 (ie AtCBP60g) has unknown function so far. In this study, Arabidopsis wild-type Columbia, knockout mutant of AtCBP60g gene (cbp60g) and homozygote of AtCBP60g overexpression lines were used as materials to check the reponses AtCBP60g on biotic and abiotic stress. The results showed:1,The T-DNA insertion site located at the second intron, and the mutant was a tanscript null knockout by northern blot analysis.2,AtCBP60g were induced by Pseudomonas syringae(virulent and avirulent), plant hormones(SA,ABA,JA),and oxidative stress.3,In wild type, cbp60g and overexpression lines OE26-1, OE17-10, the PR gene expression levels after virulent and avirulent P. syringae infection are: cbp60g