Mutation Breeding Of Streptomyces Albus By Low Energy N~+ Implantation

Salinomycin produced by Streptomyces albus is a polyether antibiotic specific for animal and is widely used as an anti-coccidia agent due to its high efficacy, broad spectrum and low residue. Though Salinomycin was initially produced in China in early 90's, the product is still lack of competitiveness because of poor quality and high cost. Nevertheless strain modification and process optimization could be used as two approaches to improve the yield and reduce the cost.The discovery of bio-effects of ion beam implantation paves a new way to improve biological varieties. In this paper, mutation breeding of Streptomyces albus with low energy ion beam was studied. The results showed that ion beam implantation could be an efficient way to improve Salinomycin. And it is significant to further research bio-effects of ion beam and expand the application of industry microbe breeding.In this paper, the function and application of Salinomycin was firstly introduced, and then the position of Salinomycin in animal medical and the function of ion beam biotechnology in research process of Salinomycin were also summarizes.Using Sterptomyces albus SL-610 as the producing strain, the yield of the fermentation could be increased largely with the mutagenesis induced by ion beam. Three new strains SL-190, SL-210, SL-230 were screened and the activities were improved 73.3%, 91.53% and 65.11% respectively. The results of physiology characteristic showed that the obtained mutant strains were genetically stable and grew fast. 30ml loading amount and 12% inoculation size were considered as the optimal condition and the optimum fermentation medium is 12% defatted wheat germ flour, 15% bean oil, 1.8% carbamide and 7.0% calcium carbonate.