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Studies On The Biology Of Carabus Smaragdinus And The Intestinal Bacteria Of C. Smaragdinus Larvae

Posted on:2010-07-12Degree:MasterType:Thesis
Country:ChinaCandidate:Z P WangFull Text:PDF
GTID:2120360278967194Subject:Agricultural Entomology and Pest Control
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Carabus smaragdinus Fischer von Waldheim belongs to Carabus Linnae, Carabidae, Coleoptrra. It is an important value of the predator of insect resource, which distributes in the most northern provinces of China , the south-eastern Siberia, far east area of Russia and Korea. It can prey on snail, limax,Locusta migratoria manilensis Meyen and many kinds Lepidoptera larvae such as the larvae of Mythimna separate Walker and Antheraea perny Guérin-Méneville etc. We could use Tenebrio molitor L., Eupolyphaga sinensis Walker and Bombyx mori L. to feed Carabus smaragdinus Fisher in artificial rearing.In this paper, the biology characteristics and the adults'intestine of C. smaragdinus were studied by reseaching 4 kinds of Carabid ground beetles resouces, as astheoretical and experimental evidence for future studies on artificial breeding and field releasing.1. By investigating Carabid ground beetles resources in Shandong province, identifying and statistical analysis, 4 species were collected. There were Carabus smaragdinus Fischer von Waldheim, Carabus brandti Faldermann, Calosoma maximoviczi Morowitzi and Carabus sternbergi Lapouge.2. C. smaragdinus was holometabolism insect, experiencing egg, larvae, pupae and adult. The adults were 32.8~36.7 mm in length, 10.3~11.3 mm in width. The head and pronotum were dirty red, elytra was green, surface-irregularities were black, with metallic sheen on the surface. Pronotum is heart-shaped nearly, with its lateral margins become narrow lightly from mid. Elytra was long oval-shaped, its basal was the same width as the pronotum. The distinction between males and females were: the tarsi of the forefoot of males was wider than females, females were bigger than males generally. Egg was yellow, long oval and slightly curved. For Larva, the body was flat, back and ventral surface were black, and had slightly blue luster. Pupa was light yellow, and the body slightly curved, and showed olive shape3. It occoured two genetations per year in Taian and overwintered as adult in earth. The adults emerged from last ten-day period of April to the last ten-day period of October, and oviposited in early June. At 25℃, eggs hatched after 5~7 days; the larvae had two instars, the 1st instar stage was 4~5 days, 2nd instar 25~28 days, and the pupal stage lasted 7~9 days at 25℃.The female adults oviposited 2~3 times every year, and lay 7~15 eggs each time. The 1st instar larvae can prey on 1~2 snails, 2nd instar larvae 7~10. One adult can prey on 3rd Locusta migratoria manilensis (Meyen) 1~2 one day, mature larvae of Tenebrio molitor L. 1~3. Mortality of experimental population of C. smaragdinus were observed at 25℃, 28℃and 30℃respectively. And their life tables at these temperatures were made. At these temperatures the survival rates were 42.42%,62.79% and 43.75% respectively.4. Twelve bacteria strains were isolated from the intestine of the adult of C. smaragdinus, and were identified as Bacillus subtilis, Klebsiella. axytoca, Actinobacillus sp., Staphylococcus sp., B. megaterium, B. coagulans, Microbacterium sp.,B. lichenifomis, B. clausii, Exigwobacterium sp.,Cellulomonas sp. and B. larrae, based on bacterial modality, dyeing reaction, cultural characteristics, physiological characteristics and biochemical reactions.
Keywords/Search Tags:Carabidae, Carabus smaragdinus Fischer, biology, intestine bacteria
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