Studies On Tissue Culture And Plant Regeneration Of Late Maturing Honey Peach

Late maturing honey peach is the excellent variety bred by Forestry Institute of Beijing Academy of Agriculture and Forestry. It has been long enjoyed for its exuberant nutrition, delicious taste, good-sized shape, compact pulp, late maturing age, easy transportation and storage, and bright market prospect. In order to accelerate the widespread implementation of the excellent variety, it is necessary to improve its quality fatherly, adopt the modern seedling-raising technique, build special germchit breeding and cultivating base, and establish effective and stable regeneration system which is fine for genetic transformation of late maturing honey peach.Through in-vitro breeding the exogenous stem, terminal bud, blade as explants, this study established the sterility system; through the combination of contrast orthogonal test and variance analysis, bred the explants, studied the plant regeneration, and probed the best sampling time, position and the best breeding medium proliferation recipe. The experiment results appear as follows:1. In the initially breeding the stem of the late maturing honey peach, the most proper breeding medium and the hormone combination that induce the growth of the late maturing honey peach stem are achieved as follows: G+6-BA4.0mg/L+ IBA0.6mg/L+GA33.0mg/L.2. In the inducing cultivation of the late maturing honey peach leaf callus, the best breeding medium recipe of the late maturing honey peach leaf callus inducing cultivation is achieved as follows: G+IBA0.1mg/L+TDZ1.5mg/L.3. In the sub-breeding the stem of the late maturing honey peach, the most proper breeding medium and the hormone combination of the late maturing honey peach proliferation cultivation are achieved as follows: G+6-BA0.25mg/L+IBA0.25mg/L +GA31.0mg/L.4. In the induction and proliferation cultivation of the multiple shoots of the late maturing honey peach, the most proper breeding medium and the hormone combination of the multiple shoots are achieved as follows: G+6-BA0.25mg/L +IBA0.25mg/L+GA30.5mg/L.5. In the induction and proliferation cultivation of the leaf callus of the late maturing honey peach, the best breeding medium recipe of the cultivation proliferation of the late maturing honey peach leaf callus are achieved as follows: G+6-BA3.0mg/L+NAA0.5mg/L.