Interspecific Relations Between Chlorella Vulgaris And Haematococcus Pluvialis Under Co-culture

Chlorella vulgaris belongs to Chlorophyta,Chlorophyceae,Chlorococcales,Oocystaceae, Chlorella.Haematococcus pluvialis belongs to Chlorophyta,Chlorophyceae,Volvocales, Haematococceae,Haematococcus.As these two economic algae are rich in proteins,lipids, polysaccharides,vitamins and activity of metabolites,they have wide application prospect and scientific research value in food,cosmetics,health products and pharmaceutical industry and other fields.The experiments were carried out into co-culture Chlorella vulgaris(C) and Haematococcus pluvialis(H) under 5 inoculative proportions(9C:1H,7C:3H,5C:5H,3C:7H,1C:9H),4 different N,P nutrient co-cultivation and allelopathic effect experiments.The results showed(1) Appropriate inoculation ratios between Chlorella vulgaris and Haematococcus pluvialis could significantly improve the growth of co-cultured system.The treatments of 7C:3H and 5C:5H apparently showed algae promotion,which cell density,biomass and chlorophyll a were much more higher than other treatments and controls.(2) N,P nutrition and its adding method had significant impact on the growth of Chlorella vulgaris and Haematococcus pluvialis. In 4 different nutrition experiment,supplemental nutrition showed the highest cell density, biomass and chlorophyll a content,especially its treatment of 5C:5H,which result of cell density were 46.3%higher than that of nomal nutrition,and biomass and chl a of 9C:1H was 77.7%and 58.4%much higher than those of nomal nutrition.(3) N,P nutrient utilization in treatment of co-culture were much higher than mono-culture.At the end period of cell growth,the treatments of 5C:5H had the highest N,P nutrient utilization,92.7%-99.7%and 98.8%-99.4%respectively. (4) There were allelopathic effect between Chlorella vulgaris and Haematococcus pluvialis. Chlorella vulgaris(donor) had promotion effect on Haematococcus pluvialis(acceptor),which growth of cell denstiy was 2.3%-15.8%.Under nomal nutrition,filtrate from Haematococcus pluvialis had promotion effect on Chlorella vulgaris,which growth of cell density was 2.0%-16.8%;however,Under limited nutrition filtrate from Haematococcus pluvialis had inhibition effect on Chlorella vulgaris,and the inhibitory ratio was 10.0%-14.1%.