Analyzed The Chinese Primary Caryota Urens Linn Heredity Multiplicity Using ISSR

Caryota urens Linn.is often green the tree,it is our country rare large-scale palm plant,the natural resource are few,mainly had been born in the elevation 370-2000m place hillside and the run-off ditch.It is the national two levels of protections is in imminent danger plants.Sequence research preliminary analysis China primary Caryota has been 4 kinds through chloroplast trnK the gene content during matK to be the phylogeny and has analyzed the Caryota urens Linn..heredity multiplicity through ISSR.Uses the CTAB method from the Caryota urens Linn..fresh leaf to try to find out and withdraw Caryota urens Linn.DNA,discovers the best extraction condition and the medicament amount used.In this foundation,carried on to Caryota urens Linn. ISSR-PCR expands increases,selected 15 primers from 100 ISSR Primers,which may expand clearly,duplicate well.15 primers amplified 109 materials to increase 163 bandings,the amplified bandings molecular weight size between 0.1-2kb,156 bandings had the polymorphism,its polymorphism percentage was 95.7%.Amplified bands number was from 5 to 17,was equally 11.The Caryota urens Linn.heredity multiplicity and differentiation coefficient obtained by the POPGENE software. Caryota urens Linn..species level heredity multiple(Ht) were 0.2846,occupied in the group to inherit multiple(Hs)is 0.1441。The Caryota urens Linn.(Gst) value was 0.4937,namely 49.37%variations existed in occupy the group,50.63%variation existed in occupying in the group,explained Caryota urens Linn.the hereditary change existed in occupying in the group with to occupy the group.Nei's heredity multiple index h was 0.2866,the Shannon's information index I was 0.4370.Obtained by the UPGMA occupies the group based on the Nei's non-weighting computation heredity from different Caryota urens Linn.population.Various communities'heredity was away from between 0.0178～0.3664.Heredity between MZ and the TS community's was away from slightly,heredity between the NP c and LS community's was away from in a big way.Explained the split up degree between the community was high.Occupied the group the UPGMA cluster result to indicate that, Caryota urens Linn.13 clusters may divide into three big class groups:The first class group including Youyiguan,Shuikouzhen,Xinlian and Nongda, in which Youyiguan and Shuikouzhen's relationship relations was near,gathers was one small tired,the new association stirs reaches gathered a minor sort.Second class group including Guangxi botanical garden,Andezhen,Napo, Shangchagnwan,Tianxin,Mengzi Landscape bureau and Maguan.This class group classification was complex,also subdivided two branches.A branch was including Guangxi botanical garden,Andezhen and Napo,another including on Shangchagnwan, Tianxin,Mengzi Landscape bureau and Maguan.The third branch was quite small,included merely Xingjie and Longshuchong two occupies in the group.Caryota urens Linn.in the group has the huge hereditary change,adaptive faculty.The rich heredity multiplicity had provided the variation fountainhead for Caryota urens Linn..The Caryota urens Linn.distribution was broad,the cultivation history was glorious,but occupies the group quite to be all small wildly,assumes the islands shape distribution pattern,proposed the Caryota urens Linn.protection strategy.Determined 4 kinds of Chinese primary Caryota matK sequence.The result showed,Caryota's matK sequence existence certain information bit spot,but more conservative;Through its matK sequence cluster analysis indicated the genealogical tree has two branches,for the Caryota,another for Caryota urens Linn.,Caryota mitis Becc and Caryota monostachya Becc,in which Caryota urens Linn.and Caryota monostachya Becc blood relationship relations is recent.This kind of classified and morphology classified have some differences.