The Formation And Regulation Mechanism Of Ectodesmata-like During The Garlic Declining

During the end of dormancy and bud germination, parenchyma cells, which from the central pore of the inner epidermis layer to outside in garlic, were gradually declined. Using the immunocytochemical technique and fluorescence microscopy combating with transmission electron microscopy observed, we discussed the mechanism of the cell-to-cell transport and changes between the declined cells and living cells during the germination. The results showed that parenchyma cells were shrinking and the extracellular spaces were expanding as result of a large number of inclusions outgoing traffic. It was described the process that the plasmodesmata were gradually stretched and broke into the ectodesmata-like, while the diameter and appressed endoplasmic reticulum in plasmodesmata became thinner. When the parenchyma tissues were pretreated by Cytochalasin D, phalloidin, 2, 3-butanedione, 2-monoxime and then combined with fluorescence probe detecting, the results showed that F-actin and myosin may take important role in regulating of the symplastic transport of the ectodesmata-like. Actin and myosin were labeled with immuno-gold within plasmodesmata and ectodesmata-like and demonstrated that they were both as an important component and controlling the material transport. Using fluorescence probe LYCH and the aniline blue staining, callose may take part in regulating between the declined cells and living cells interface and maintaining the function of inner cells normal physiological activities after the tissue were sliced and incubated with different times.