Screening Mutant Of Nitric Oxide Sno1 In Arabidopsis And The Mapping Of The Genetic Locus

Nitric oxide(NO) is a diatomic gas that performs crucial functions in a wide array of physiological processes in plants.It was undoubtedly an era of great enthusiasm for NO as a plant signaling molecule and,today,it is known to play a crucial role in the regulation of physiological processes ranging from development to adaptation to biotic and abiotic stresses.In recent years,although the research on NO function in plant has made substantial progress,the molecular mechanisms underlying its effects remain poorly understood,esepecialy,in the level of genes.We have isolated sevel NO-sensitive mutants by forward genetics,and snol is one of them.When treated with 50uM sodium-nitroprussiate(SNP),an NO donor,the root of snol is inhibited more clearly than wild type.It shows that the snol mutant is more sensitive to NO than the wild type.Moreover,we find that snol is also very sensitive to KCl by the physiological biochemistry experiment.We determinate nine ions content in mutant snol and wild type,which treated with different concentration of SNP,and we also determinate ion content in mutant noxl and Atnoal.All the results prove that NO can inhibit the absorbtion of the K for the mutant snol.The reason is that the mutant gene may affect the homeostasis of K in plants,and then affect the growth of plant roots.In addition,genetic analysis show that the mutant is caused by single recessive gene mutation.And we position the mutation to a segment of 20kb on the 5th chromosome in Arabidopsis by the map-based cloning technique.There are five genes in the segment and we are sequencing it.