Storage Of Pavlova Viridis By Encapsulation-dehydration Under Normal Temperature And Low Temperature

Plant germplasm resources for the survival of mankind and the development of the material basis for sustainable agricultural development are the material basis. For a long time, because of the diversity of plant germplasm resources and maintain the lack of sufficient attention to the crisis facing germplasm[1].Plant germplasm resources for the preservation of biodiversity conservation and plant biotechnology are of great significance [2]. Micro-propagation and restrictions on the use of tissue culture techniques, such as preservation of plant germplasm, its biggest advantage is the necessary equipment and conventional tissue culture laboratory is no different from the culture medium, culture environment oxygen content, temperature, moisture content or material change slightly , We can significantly extend the period following the use of cryopreservation freezing technology for long-term stability and preservation of plant germplasm resources to provide an effective approach, but for various reasons most of the current method is based on the study, from the practical applications still some distance [3]. At present, there are a variety of preservation methods microalgae, each method has its own advantages.Following the preservation of a simple, in the production and scientific research in practice is still favored; fixed so that preservation of the slow rate of growth of algae, can be used for secondary metabolites Production and sewage treatment; cryopreservation of germplasm maintain genetic stability, the algae species to the survival of the fittest, to facilitate long-term preservation, to minimize pollution, and other advantages [4]. Encapsulation-dehydration at room temperature and low temperature is kept constant and drying technology combined with conventional compared to save the subculture, it's time for more long-term preservation; compared with cryopreservation, it does not need more complex technology and operations The preservation of valuable equipment, and can save a lot of algae.Pavlova viridis was preserved by encapsulation-dehydration under normal temperature and low temperature. Algal cells in early stationary phase were encapsulated in 3% Ca-alginate beads with 30‰NaCl,50 million cells in one bead. Beads were desiccated with silica gel , then were preserved under normal temperature and low temperature. The main factors influencing the cell viability,such as temperature,light(dark) and water content of beads were studied. The results are as follows:1. 20℃in the dark, after 18 months, the highest survival rate of the preservation was 7.1 percent; 20℃in the light, after six months, the highest survival rate of the preservation was 8.8 percent.2. 4℃in the dark, after 18 months, the highest survival rate of the preservation was 54.1 percent; 4℃in the light, after six months, the highest survival rate of the preservation was 69.0 percent.3. -30℃, after 12 months, the highest survival rate of the preservation was 6.3 percent; after 10 percent glycerol after kept in -30℃, after 12 months, the highest survival rate was 59.7 percent.4. In this paper, respectively retained for 6 months and 18 months of algae cells were measured the growth curve and its growth rate. Save six months later, in the tenth day of the highest cell density, the average growth rate from 0.221 before the preservation reduced to 0.168, after two cycles of re-culturing, an average growth rate of 0.211. Save 18 months later, in the twelfth day of the highest cell density, the average growth rate was 0.138, after two cycles of re-culturing, the average growth rate was 0.193. After that save, the algae cells can be normal growth and reproduction.Compared with other methods, encapsulation - dehydration method is simple, no expensive equipment, the algae germplasm conservation in the broad prospects.