Detection Of Coxsackie Virus B3 By A Real-Time PCR

Golden monkey is one of the most valuable wild animals in the world. In September 2005, one monkey was suddenly dead in Changchun zoo. In order to determine the cause of death of this golden monkey, necropsies isolation of virus, and genetic test were used. According to all kinds of results, the pathogen was identified Coxsackie virus B3. CVB3, a member of the Enterovirus genus in the Picornaviridae family, is one of the most common pathogens in humans. They are associated with a wide spectrum of human illness ranging in severity from subclinical infection to rapidly fatal disease, with symptoms as diverse as acute myocarditis, aseptic meningitis, encephalitis, Bornholm disease (epidemic pleurodynia), non-specifc rashes and febrile illness. In most cases, the virus penetrates through the intestinal tract before spreading to target organs via the blood circulation. The transmission of coxsackievirus B3 occurs mainly via the oral route. To further understand the mechanism of virus persistence and its relationship to organ pathology, viral RNA kinetics in the heart and other target organs during acute phases of murine coxsackievirus B3 infection were investigated. The Detection Method for CVB3 utilizing Real-time PCR was established at first. Then BALB/c mice were inoculated by gavage and inoculated intraperitoneally with coxsackievirus B3 isolated from Sichuan golden monkey. The kinetics of CVB3 infection in the murine during acute phases was studied by quantifying viral RNA levels in the some tissues at various time-points from day 1 to day10 p.i. During the course of virus infection, viral RNA levels in all tissues peaked at day 4 p.i. then declined.Through this study, the results indicated that murine tissues infected by CVB3 can be able to detect the viral RNA. The goal of t research is to describe and understand molecular mechanisms of host-pathogen interactions in order to improve current therapies and to develop new strategies for the prevention and cure of this infectious disease. This study possesses very important significance and clinical application value in the aspect of medical research area.