The Influence Of The QDs' Modifying Groups On The Interaction Of QDs And BSA

Fluorescence probe technique is an important means for the research of the composition of cell sub-region, sub-structure and the interaction of proteins. Since the late 1970s, quantum dots (QDs) fluorescence labeling technique has aroused great attention to the people.Currently, a key point of restricting the application of quantum dots labeling technique to biomedical analysis turns to be the connection between QDs and specific biological molecules. The effective labeling between QDs and proteins is influenced by whether the linking is successful, firm, and efficient.The two main methods of the connection between QDs and biological molecules are covalent coupling and electrostatic adsorption. Covalently coupling may be solid, but generally requires more steps, and is not conducive to real-time in vivo monitoring, it may affect the surface structure of biological molecules too. Thus, the issue uses the method of electrostatic adsorption.We used sodium thioglycolate and cysteamine respectively to replace TOPO in the surface of CdSe/ZnS quantum dots, so the hydrophobic quantum dots can change into hydrophilic ones and modified by carboxy groups and amino groups. Then through electrostatic adsorption, bovine serum albumin (BSA) was connected with quantum dots. Here, we focus on the influence of the QDs' modifying group on the combination of QD and BSA.Based on the enhancement effect that the fluorescence intensity of QDs will significantly increase in the presence of trace protein (BSA), a new fluorescence scan analysis was developed for the determination of BSA.