The Partial Biological Effects In A₅₄₉ Cells Transfected Of Separase CDNA

Background:The chromosome is the elementary structure which ensures genetic materials transmit stabily,integraly and correctly.The two sister chromatids connect each other only at centromere during the mitosis metaphase and the main structural protein of centromere--cohesin is degraded during the mitosis anaphase.The two sister chromatids are segregated by spindle finber traction and allocted to two daughter cells.The key point molecule in cohesion degradation is separase which was determined in 1998.As its inhititor-Securin was degraded via ubiquitylation by the APC (anaphase-promoting complex) during the mitosis anaphase,separase once depressed by securin was released and split the cohesin's subunit(Scc1/Mcd1) resulting in the centromere cleaved and distribution of two sister chromatids.Otherwise,it will lead to aberration of chromosome in daughter cells.The change of chromosome number is one of the characteristics of tumor cells,which also affect many malignant biological phenotype including abnormality in proliferation,apoptosis and anchorage dependence.Although the research at present for the mechanism of numerical aberration of chromosome and for the relation to other malignant phenotype in tumor cells is rare,we have already determine that up-regualiation of separase can reduce the chromosomal ploidy in SPC-A cells,that its apotosis and proliferation are also affected.In our study,we observe the change of chromosomal ploid,apoptosis,proliferation and the ability of colony formation in soft ager further.In our reseach,we observed the descent in chromosomal ploid,the augment in sensitivity to apoptosis induced by cultivation without blood serum,the inhibition of malignant proliferation and the change of anchorage independence when separase was up-regulated in A₅₄₉ cells.