LncRNA TIF Regulates The Apoptosis Of A549 Cells Through OMA1

Objective: The purpose of this article is to study the effect of lncRNA TIF on Doxorubicininduced apoptosis of A549 cells and to explore the mechanism of lncRNA TIF on the apoptosis of A549 cells.This might provide a new perspective for the treatment of lung cancer.Method:(1)By high-throughput lncRNAs microarray technique,differential expression of lncRNAs in the 2?M doxorubicin treated group was screened,and then the significantly differentially expressed lncRNAs were verifed by real-time quantitative PCR(RT-qPCR).(2)The expression of lncRNA TIF in 19 pairs lung tumor samples and normal samples was detected.(3)The lentivirus over-expression and knockdown of cell lines of lncRNA TIF were constructed successfully.(4)Mitochondrial division and fusion were detected by Mito Tracker.(5)The apoptosis of cells was detected through flow cytometry.(6)Migration of the cell was detected by scratch test.(7)The relationship between lncRNA TIF and mitochondrial division protein OMA1 was explored by RT-qPCR and western blot.Result:(1)According to the results of high-throughput lncRNAs microarray and RTqPCR,lnc-ZMYM6-2-1,we named lncRNA TIF(Tumor Inhibitor Factor)was selected as the candidate lncRNA.(2)The expression of lncRNA TIF was down-regulated in the lung tumor samples.(3)Doxorubicin can promote the mitochondrial division of A549 cells,but knock down lncRNA TIF can inhibit doxorubicin-induced mitochondrial division.(4)Doxorubicin can promote the apoptosis of A549 cells,but knock down lncRNA TIF inhibits Doxorubicin-induced apoptosis.(5)Knock down lncRNA TIF promotes migration of A549 cells.(6)The expression of OMA1 at mRNA and protein levels was upregulated when over-expression lncRNA TIF;the expression of OMA1 in mRNA and protein levels was down-regulated when knock down the expression of lncRNA TIF.(7)Doxorubicin can induce the expression of OMA1 in mRNA and protein levels.Conclusion: The expression of lncRNA TIF was down-regulated in the lung tumor samples,but the expression of lncRNA TIF was up-regulated in the doxorubicin treated group;lncRNA TIF can promote the apoptosis of A549 cells and inhibit it's migration;OMA1 has the function of promoting mitochondrial division and apoptosis,and the expression of OMA1 was up-regulated in the doxorubicin treated group;The expression of lncRNA TIF was positively correlated with the expression of OMA1.Therefore,lncRNA TIF plays an important role in the apoptosis process of A549 cells.For this reason,lncRNA TIF may provide a new perspective for the treatment of clinical lung cancer as a new target molecule.