Genetic Analysis And Gene Mapping Of A Mutants Involved In Reproductive Development In Rice

The acquisition and research on the rice mutants related to reproductive development played an important role in uncovering the function and interaction of genes in reproductive process,especially in floral development.Research on rice reproductive mechanism is significant both in theory and in human agriculture.The mutant strains called in this paper frol(function of reproductive organ 1)were found by our research group,and the genes involved were named as frol(t)respectively. They were found in T2 population of minghui86 transgenic rice progeny.We have conducted researches on frol in three aspects:morphological observation,genetic analysis and gene mapping,setting strong foundation for gene expressing and gene clonin Genetic analysis of non-palesMutant frol had heading but not flowering.Floret shape of frol mutant is similar to that of tiny green capsicum,and in which stamens and pistils are abnormal, translucent and sterile.Four F2 populations,Minghui86/ mutant,R527/ mutant,93-11/ mutant and Zhonghua16/ mutant were developed,the result of genetic analysisindicated that the mutant is controlled by a single recessive gene in the near future.Using mutant heterozygotes of frol,F2 populations were developed from the crosses of frol and zhonghua16 to detect molecular markers linked with the gene of frol(t),with the aid of SSR mark technique and by means of BSA analysis.Finally,the frol(t)was mapped between marker RM23432 and maker inDe10802 in the region of chromsome8.RM23432 was on one side of frol(t)with genetic distance of 1.4 cM,and inDe10802 was on the other side with 0.6 cM respectively.