RAPD Analysis Of Genetic Diversity Of Salix Gordejevii In Inner Mongolia

Salix gordejevii (Salicaceae) was a kind of dune-fixing pioneer shrub in sandland and had characteristics of resistance to drought tolerance, wind erosion and sand-buring. It was not only the constructive species and dominant species in moving and semi-fixed dunes, but also had higher genetic and ecological value. Random amplified polymorphic DNA (RAPD) was applied to detect genetic diversity of four populations of Salix gordejevii in Inner Mongolia. Through the amplification with 13 random primers, 153 repeatable loci in which 128 were polymorphic. Percentage of polymorphic loci were 83.66%. The results were as follows: percentage of polymorphic loci was between 88.24%and 96.08% in populations. Among them, population of the Hulunbeier Sandland was the highest and the population of Hunshandak Sandland was lowest of percentage of polymorphic loci. According to the genetic diversity of populations by Shannon's Information Index (I) and Nei's gene diversity (h), the ranking among them were the population of Hulunbeier Sandland > the population of easten Horqin Sandland > the population of westen Horqin Sandland >the population of Hunshandak Sandland. Genetic variation existed mainly within populations (94.27%) rather than among populations by Nei's gene diversity. The gene flow among populations were very high, Nm=11.31. Nei's genetic identity respectively were 0.8961 and 0.9543 among populations. The result revealed that 4 populations were related. Using unweighted pair group method with arithmetic average (UPGMA), 4 populations of Salix gordejevii were clustered into three groups, population of Easten Horqin Sandland and population of Westen Horqin Sandyland got together first, then population of Hunshandak Sandland joined in, and the final population was Hulunbeier Sandland. There was significantly correlation between genetic distance and geographical distance (P<0.01). Environment factors in each sampling situs including altitude, meant annual temperature and annual precipitation and so on, had not significant correlation with gene diversity of Salix gordejevii.