Temporal-Spatial Expression Of SHH And β-Catenin During Hair Follicle Morphogenesis Of Different Hoof Periphery Site In Bos Grunniens Embryos

Ed(embryonic day)45～248 Yak embryos hoof periphery skin and Ed 55～275 the cattle's were used as test and control material respectively to investigate hair follicle morphogenesis of different hoof periphery site, the paraffin embed tissue section was stained by H.E method for studying the histomorphogenesis changes and EnVision immunohistochemistry for detecting qualitatively and quantitatively temprocal-spatial expression of SHH andβ-catenin during hair follicle morphogenesis,Results is following:1. The hair follicle morphogenesis of yak proximal hoof end and distal hoof end initiate from Ed 45 and initiate from Ed 55 respectively, mature hair follicle in Ed 122. From the proximal hoof end to distal hoof end, the time ,the phase,the size and the growing depth of the hair follicle morphogenesis in turn occure degressive gradient. There are secondary hair follicles and hair follicle groups in Yak hoof periphery area.2. SHH andβ-catenin expression of hoof periphery hair follicle morphogenesis is related with different hoof periphery site, signaling wave is transmitted from the proximal hoof end to distal hoof end in yak. However, SHH andβ-catenin expression of cattle hoof periphery hair follicle morphogenesis is no related with different hoof periphery site, the signaling transmit pattern is different from that yak work.3. SHH andβ-catenin expression of hoof periphery hair follicle morphogenesis in yak and cattle embryos always is coupled with hair follicle morphogenesis phases, whereas the expression intensity of SHH andβ-catenin in yak is much higher than cattle's.4. SHH expression during hair follicle morphogenesis show stronger effect on the hair follicle epidermis than the hair follicle mesenchyme..5.β-catenin expression during hair follicle morphogenesis show stronger effect on the hair follicle epidermis than the hair follicle mesenchyme.Our results suggest SHH andβ-catenin cooperate each other and respective function during the hoof periphery hair follicle morphogenesis .SHH is responsible for directing differentiation and determining cell fate ,β-catenin regulate differentiated cell proliferation .We speculate on SHH andβ-catenin expression and detail function difference play important role during hair follicle morphogenesis in different hoof periphery site of yak embryos. The epidermis dominate during the hair follicle morphogenesis and the mesenchyme signaling is secondary. Intensity difference ofβ-catenin expression in yak and cattle may act as key factor to determine the hair follicle size gradient.