Construction Of Bacterial Artificial Chromosome Library Of Chinese Alligator (Alligator Sinensis)

The Chinese Alligator (Alligator sinensis) is rare and endangered species endemic to China. Due to environmental deterioration and habitat loss, the population of the Chinese Alligator declined rapidly. In order to protect the rare and endangered species, the research about Chinese Alligator conservation has begun. Here we constructed a high-quality bacterial artificial chromosome (BAC) library of the Chinese Alligator with fresh blood, which was provided by Changxing Research base of Chinese Alligator breeding. The library consists of 164 640 clones, which are arrayed in 1 715 96-well microtiter plates. By insert size testing of 98 clones randomly picked from the library, the average insert size of the BAC clones is estimated to be 96 kb and non-insert clones about 4.08%. As a reference of the genome size of American Alligator, the Chinese Alligator BAC library contains about 6-fold genome coverage. The probability of any Chinese Alligator gene being found in this library should be about 99.71%.