| Chinese alligator (Alligator sinensis) is a endemic rare species in China and also a most threatened one of Crocodilian. For Chinese alligator's conservation, genetic conservation is as important as habitat conservation. Genetic variation is major study content in conservation genetics. In small population, genetic variation loss because of bottleneck effect, inbreeding, founder effect, and genetic drift. Control region sequence of Mitochondrial DNA is good genetic markers for population genetic structure study, with amplification and analysis of control region sequences of Chinese alligator individuals we found only four haplotypes appear in the 64 specimens and Chinese alligator is at low genetic variation level not only in captive breeding population but also in wild individuals ( π =0.00024±0.00016) , which indicates that the rare species has experienced a strong bottleneck effect in history. Reintroduction plan for Chinese alligator has been performed and genetic management for released individuals become study content. We analysis the genetic similarity of 13 pre-selective Chinese alligator for reintroduction experiment, with mtDNA control region sequence and 10 pairs microsatellite primers of American alligator (Alligator mississippiensis). Result indicates that the control region sequences has low resolution for genetic similarity and microsatellite loci of American alligator is not effective for Chinese alligator, just 2 pair of primers are polymorphism and suitable for genetic analysis. To own enough mircosatellite loci for genetic analysis of Chinese alligator, we isolate 17 loci from Chinese alligator genomics with enrichment library method.
|
PDF Full Text Request