Study On Vitro And Secretory Structure Of Tube Seedling Of Artemisia Selengensis

Artemisia selengensis.is the unique aquatic perennial herb of the artemisia which is distributed in the provinces of the northeastern,the north china,central china,and the southwestern,the PoYang lake and the Dong Ting lake is the main productive area.An upsurge of ingestion and exploitation appeared especially after the 1980s. The young stem of Artemisia selengensis is not only very delicious,but also have abundant nutrient element,which is regarded as a delicacy of the dinner.Besides,it can be used as medicine as well.And it is a precious plant resource that nature bestowed on human being.For a better protection of this plants as well as a sustainable development and exploration,it's imperative to build a new technical platform for Preservation on germplasm of Artemisia selengensis,Variety Improvement and Industrialization.Besides,Artemisia selengensis has a special flavor of Artemisia volatile oil related closely to the structure.this paper begin with the culture in vitro of Artemisia selengensis in Yunnan,build regenerated system of culture in vitro,study secretory structure and development on tube seedling of Artemisia selengensis,Which fill the blank with no reports about secretory structure of Artemisia selengensis.The main results of this study are as follows:1.Build culture in vitro regenerating system which take Artemisia selengensis of Yunnan as the test material.The expressive ability about cell all-roundness of Yunnan Artemisia selengensis is very strong.It is suitable for inducing of the rapid and intense embryo callus in MS+BA1mg·L^-1+2.4-D4mg·L^-1culture medium;It is suitable for differentiation and multiplication of the bud in MS+BA1mg·L^-1+NAA0.4mg·L^-1culture medium;It is suitable for the in vitro seedling striking root and forming rhizome in 1/2MS+NAA0.5mg·L^-1+IAA0.5mg·L^-1culture medium.2.If explants of Yunnan Artemisia selengensis are different,the colour and texture of the callus that explants induced are universally different.Generally,the callus induced by the young stems are pistachio embryo callus; the callus induced by young leaves are deep green,mauve,purplish red,etc.The various callus lay afford a good foundation for selecting mutations of Artemisia Selengensis3.Through cytohistology observation,it was found that there are some differences with callus dedifferentiation and different values,and there were two kind of source of the young stem callus.The first kind is dedifferentiated from the cortical parenchyma cells of the epidermis and its below;the second kind is dedifferentiated from the parenchyma cells of the basic tissue around the vascular tissue at the incision.The young leaves callus mainly come from meristematic cells which dedifferentiate from a part of mesophyll cells,they are enveloped with the callus from the leaf margin to the whole explants.In the meanwhile,a large proportion of mesophyll cells protoplasts autolyzes,cell walls thickening into the tracheary elements,which is the character sympolizing the differentiation of the mesophyll cells.The young callus of Artemisia selengensis appeared with structure of embryogenic cells,embryogenic cell masses and meristematic nodules,but the embryogenic cells is predominant of them,so the subculture capacity of Artemisia selengensis callus is strong.Differentiation of the bud of Artemisia selengensis callu belongs to external origination.In rooting cultivation of the tube seedling,the root primordia is originated from the pith rays of the cortex,which is formed by the meristematic cells that dedifferentiate from the parenchyma cells.This is endogenesis in origin.4.There are two secretory cavities and glands in Tube seedling of Artemisia selengensis,Which Provides a new way to study systemly structure and development of plant secretory under the artificial conditions.Secretory cavities of Artemisia selengensis are cleft secretion cavity,Which are distributed in cortex of Stem and rhizome.And it is a group cell with working closely, Among which the middle class dissolved and disappear,Cell mutually separated and passaged outward,formed a spherical cavity which is arounded by a layer of Epithelial cells with secretion capacity in secretory cavitiesThere are two kind of glands in the young stemand young leaf of tube seedling of Artemisia selengensis.One is glandularia gland which consist of 10 cells,2 basal cells, 2 stalk cells,6 glandularia cells,they arrange in 2 rows.It was sectorial cystic when matured.The other one is non- glandularia gland which consist of tubular gland cell.After systematic observation of the glandularia gland,we found that they are diversified,besides 2-row-arranged,they also appeared paracytic and 3-row-arranged.Some glandularia cells consist of overlOcells,We still need further study about how on paracytic cells decelop.