Molecular Identification And Genetic Relationships Of Ectomycorrhizal Fungus Suillus Strains Isolated From Inner Mongolia

The molecular identification and genetic diversity of 27 Suillus strains from different hosts and different sites were studied based on the sequencing analysis of ITS, the internal transcribed spacer region of the ribosomal RNA genes, and ITS-RFLP. The phylogenetic analysis established the close relationship among these Suillus strains. ITS sequences of 27 Suillus strains were subjected to a GneBank search and SP1,SP2,SP3,SP4,SP5,SP6,SP7,SP8,SP9,SF1,SF2,SF3,P.Lac-1,P.Lac-2,3ˊ,3″,3# were belonged to Suillus luteus with 99% similarity to DQ440568.1 in GneBank. 27 Suillus strains were classified into 3 genotypes according to the percent identity of sequence and were divided into 2 patterns of ITS digested with 3 restriction endonuclease. The results of the sequencing multiple sequence alignment analysis and the results of ITS-RFLP were similar. The great genetic distance of"zhang2"and 1" from others may be the results of the influence by its hosts and habitats. Although sequence analysis of ITS is accurate, the ITS-RFLP is simple and practical and may be useful to the quick identification of ectomycorrhizal fungi. It is more effective and accurative for using these two techniques.