The Exploration And Research Of Human And Horse ACE Gene Polymorphism And Resistance

Total DNA of equine blood was extracted. According to the 16,17 extron of ACE gene of people,sheep,rabbit published in GenBank, a pairs of conservative primers was designed and named p1 . Ddestination gene was amplified by PCR and was cloned into PMD19-T vector and then sequenced. The acquired nucleotide sequences were analysed by DNAStar software. As a results, the acquired sequences was 345bp long, and then compared with the counterpart sequences of human ACE gene's 16～17 extron (11576～12060bp) sequence. They nucleotide homology were 82.3%. This proved that the destination gene include ACE gene 16 intron sequence. According to the acquired sequences,a pairs of specificity primers was designed and named P2, ACE gene of five kinds horses was amplified by PCR and Compaired with human ACE gene .The result showed that horse ACE gene had no polymorphism. This resource work has provided a wide range of information not only for the study of horse ACE gene polymorphism, but also for the choose of horse racing.