| The genus of Odorrana belongs to Ranidae Family (Amphibia Class, Anura Order). It was classied as Luctuosa Group in Rana Genus, according to the character of the species, regarded as the important transition group from real frog to water frog. Rana Luctuosa Group was seperated from Rana Genus which was set up a new genus—Odorrana according to the common character of the Luctuosa Group and comparded with the character of type species of Rana . The genus Odorrana includes about 24 species, which distributes in subtropic and tropic of Asia, China has 19 species and 8 species are in Guizhou Province. The taxonomy and phylogeny of several species of Odorrana in Guizhou were reviewed about morphology and molecule. 1.Study on morphology:7 species in Guizhou were studied by cluster and discriminant analysis. The two ways can distinguish some species: built discriminant function of all species by discriminant analysis: studied on the similar degree by cluster analysis. Discussed the class and status of O. margaretae in Xing Yi, Pan Xian,An Long and Wen Ning.The results indicated that: 7 species were very diffrent in the morphology but a bit similar. The result showed that O. margaretae,O. lungshengensis,O. junlianensis were similar otherwise O. anlungnsis and O. wuchuanensis which are special species in Guizhou were different from others. Discriminant analysis also proved it. Different populations of O. margaretae were not the same in the morphology but had the great similarity and they were not departed completed by Discriminant analysis; Cluster analysis showed that 6 populations of O. margaretae could classified 3 typies:type I included An Long and Wei Ning population;Xing Yi and Pan Xian population belonged to type II,type III included Guan Xian and Wu Shan population; Type I was the transition type of type II and type III, with the result of the consistency of the geographical distribution.2 .Study on molecule:Fragments of mitochondrial 12S and 16S rRNA genes of 18 individuals were sequenced, including 9 species of Odorrana and 6 populations O. margaretae used Rana nigromaculata and Fejervarya limnocharis as outgroup, results pairwised by Clustal W showed that there were about 865bp in length. The phylogenetic relationships of these taxa were analyzed by using programs of Neighbor-Joining and Maximum Parsimony: the ingroup were clustered together as one clade and O. livida diverged from the other taxa firstly;O. grahami ,O. margaretae and O. junlianensis clustered together and O. hejiangensis,O. jingdongensis and new species formed together,one consisting of different populations of O. margaretae.These results showed that different populations of O. margaretae in Panxian,Anlong ,Xing yi ,Wenning, Kuan Kuoshui, Nanjiang of Sichuan Province, Wushan of Chongqing and so on belong a species; O. grahami and O. junlianensis were a species with different names, O. jingdongensis and O. hejiangensis might a species; It was possible that O. wuchuanensis belonged to O. schumackeri group because of the difference in genetic distance.3 . Influence of the environmental factors on geographical distribution:We extracted data about environmental factors of Guizhou Odorrana species at known distribution points by using GIS, and conducted PCA analyses to evaluate impacts of these environmental factors on geographical distribution Our results show that impacts of these environmental factors on geographical distributions of Odorrana species are in order of July average temperature, annual average temperature, January average temperature, annual rainfall, annual sunshine hours and altitude, however , annual sunshine hours and altitude are of negative impact .
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