| In order to investigate the effect of IFN-γon the expression of TNF-αin the hypothala- mus-pituitary-ovary axis (HPOA axis)of RU486-induced aborted SD rats, RU486 was used to establish the abortion model, then the aborted rats were administrated with different doses of IFN-γintraperitoneal injection, the method of immunohistochemical ultrasensitive SP was us- ed to study the variation of positive TNF-αcells in hypothalamus, pituitary, ovary and uterus in normal pregnant group A, aborted model group B and three different doses of IFN-γgroup C,D,E. The main goal of this research was to study whether the low dose of IFN-γcould have a protective function to pregnancy and the mechanism of its function so as to provide morph- ologic basis for the scientific using of IFN-γin the process of animal abortion control. The results were as follows:1. The anatomical variation of uteruses in each group was observed, we found that the uteruses in group A had a pink color, and the shape of them seemed like a string of beads, congestions didn't existed in this group; the other four groups were all aborted to different extents, the color of uteruses in group B, D, E were purple black, embryos were small than the ones in group A, or the uteruses had a bamboo-shaped figuration, all embryos were aborted, uterine cavity had congestions, vagina also had bleeding situation; the color of uteruses in group C was incarnadine, some of the embryos had been eliminated, vagina had a slight extent bleeding. In conclusion, the pregnant result of group C was better than group B, D, E.2. The positive substance of TNF-αin group B was significantly increased than group A in the hypothalamus nuclei, neurohypophsis, pars intermedia, ovary follicle, corpus luteum, however, the expression of TNF-αwas obviously decreased in adenohypophysis. In the ovary stroma and uterus, TNF-αimmunopositive substance was similar with group A. It showed that expression of TNF-αimmunopositive substance in the HPOA axis of aborted rats would be enhanced to some extent when compared with the normal pregnant rats, the abnorml upregulation of TNF-αwould do no favor to pregnancy maintenance because of its belonging to TH1 cytokine.3. When aborted rats were treated with 1 IU/g IFN-γ, the change of TNF-αimmunoposi- tive substance in HPOA axis in group C were as follows: Compared with group A, expression of TNF-αin nucleus pre-opticus suprachiasmaticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus arcuatus hypothalami was significantly strengthened, conversely, the adrenohypothysis, corpus luteum, ovary stroma had a downregulating tendency; Compared with group B, 1 IU/g IFN-γcould remarkably decreased TNF-αexpression in HPOA axis of aborted rats in group C. Hence, we draw a conclusion that when IFN-γwas in the dose of 1 IU/g, it would extenuate the expression of TNF-αin the reproductive axis, this would be benefit to the pregnancy.4. When aborted rats were injected with 10 IU/g IFN-γin group D, compared with group A, expression of TNF-αinmmuopositive substance in the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami were highly increased, conversely, the ones in the nucleus pre-opticus suprachiasmaticus, neurohypophsis, adenohypophysis, ovary were obviously decreased, the change in the uterus was not remarkable; Compared with group B, expression of TNF-αin the arcuatus hypothalami was obviously enhanced, while in the nucleus pre-opticus suprachiasmaticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, neurohypophsis, pars intermedia, ovary, the results were on the contrary, the uterus had no significantly variation; Compared with group C, expression of TNF-αin the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and pars intermedia had an obviously risen trend, however, the variation in the nucleus pre-opticus suprachiasmaticus, nucleus periventricularis hypothalami, neurohypophsis, pars intermedia, follicle and corpus luteum was completely opposite, uterus had no obviously change. It indicated that the dose of 10 IU/g IFN-γcould decrease the expression of TNF-αin some nuclei of hypothalamus, some parts of pituitary and ovary, however, this function was not stronger than the dose of 1 IU/g IFN-γ.5. When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant. According to what had been mentioned above, the dose of 50 IU/g IFN-γcould not only decrease the expression of TNF-αin some parts of HPOA axis, but also increase TNF-αexpression in the other parts of HPOA axis, the function of its abated effect was not more powerful than the other two doses, this dose would do no good to the maintenence of pregnancy.
|
PDF Full Text Request