| Pinguiococcus pyrenoidosus belong to Pinguiophyceae, Pinguiococcus genara. Thepercentage of rude lipid content in this algae was 27.45％, among which EPA and saturatedfatty acids (14:0, 16:0) is high, but the contents of other kinds of fatty acids are low. SoPinguiococcus pyrenoidosus was a good material for producing PUFAs and saturated fatty acids.The effects of carbon resource(inorganic carbon compounds and organic carbon compounds)onthe growth and fatty acid content in P. pyrenoidosus CCMP 2078 are investigated in this work.Results indicate:(1) When NaHCO3 was added in one time, P. pyrenoidosus CCMP 2078 grew fastest when Cconcentration was at 5mM.; the growth of the algae was inhibited significantly when the Cconcentration was above 20mM. The total lipid acids content reached the highest value(29.7％)when NaHCO3 concentration was 5mM. Saturated fatty acids content reached the highest whenC concentration was 20mM, the absolute content of saturated fatty acids reached the highestwhen NaHCO3 concentration was 5mM. EPA and PUFAs content, EPA absolute contentdecreased as using NaHCO3 as carbon resource.(2) NaHCO3 was added equally in several times, when NaHCO3 concentration was 20mM, celldensity reached the highest value(8.93×106 cells/ml), when NaHCO3 concentration was 10mM,the total lipid acids content, EPA and PUFAs content are all the lowest (21.2％, 8.2％and 19.26％respectively), saturated fatty acids content and absolute content reached the highest(21.2％,32.2mg/L respectively). EPA content and absolute content reached the highest when NaHCO3concentration was 20mM.(3) When CO2 was used as carbon resource, P. pyrenoidosus CCMP 2078 grew fastest when CO2content was 0.5％, the total lipid acids content reached the highest value(21.3％), the highest EPAand saturated fatty acids absolute content reached(17.62 mg/L, 46.3 mg/L respectively), whenCO2 content was 1％, the content of 16:0 reached 63.78％. The percentage content of EPA andPUFAs decreased by adding CO2;(4) The algae grew well in the range of 5 to 40g/L of glucose. The percentage content of EPAand PUFAs and EPA absolute content reached the highest value (39.65％, 69.46％and 16.2mg/Lrespectively) at 5g/L glucose. When glucose content was 40g/L, the percentage content ofsaturated fatty acids and absolute content reached the highest(56.9％, 18.86mg/L respectively). (5) P. pyrenoidosus CCMP 2078 grew fastest when glycin content was 0.5g/L, The growth of thealgae was inhibited significantly when the glycin concentration increased to 3g/L. When glycinconcentration was 1g/L, the total lipid acids content reached the highest value (31.3％), and thepercentage content of saturated fatty acids and absolute content reached the highest. Within therange of 0.5g/L to 3g/L, the percentage content of EPA and PUFAs increased as the glycinconcentration raised. EPA absolute content had no change as using glycin as carbon resource.(6) P. pyrenoidosus CCMP 2078 grew best when glycerin concentration was 10g/L and the dryweight reached to 0.303g/L. The total lipid acids content,saturated fatty acids and EPA％contents decreased caused by adding glycerin; Within the range of 0g/L to 20g/L, PUFAs％contents decreased as the glycerin concentration raised; EPA absolute content reached thehighest at glycerin 10g/L. The absolute content of saturated fatty acids reached thehighest(18.86mg/L), when glycerin concentration was 20g/L.(7) P. pyrenoidosus CCMP 2078 grew well within the range of sodium acetate concentration from0.5g/L to 3g/L, it grew fastest when sodium acetate concentration was 0.5g/L. The total lipid acidscontent, the percentage content of saturated fatty acids, EPA and PUFAs, EPA and saturated fattyacids absolute content decreased as using sodium acetate as carbon resource.
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