Molercular Cloning And Characterization Of CesA Gene In Catharanthus Roseus Leaves Under Different Grain Diameter Of Soil

In this study, we used the Catharanthus Roseus as the material to discuss the cloning and characterization of cellulose synthetical gene under different grain diameter and water content of soil. We got the 476bp fragment of CesA gene by RT-PCR. The result of Northern blotting indicated that the expression of CesA gene lower under the stress of water than under the normal when the level of soil grain diameter was invariable. Under the same water stress and different soil grain diameter the expression of CesA gene was higher in the situation of 60%A＋40%B and 40%A＋60%B. when the water was normal, there was not obvious distinction. In addition, we analysised the relation between endogenesis hormone and the expression of CesA gene. This work would be a theoretical basis for the further study of Cellulose synthesis and regulation, and would be helpful to the study of plant response under stress.The main results as fellow:1. The grain diameter, compaction and water content of soil were distinct in different condition.2. In this study, we purified RNA from Catharanthus Roseus with TRIZOL, and tested RNA by agarose gel. RT-PCR useing RNA as a template, we got the 476bp fragment of CesA gene. The homology of it with eucalypt, Hordeum Vulgare is 99%.3. Northern blotting could discuss the expression of CesA gene under different grain diameter and water content of soil. We can see that, the expression of CesA gene lower under the stress of water than under the normal when the leve of soil grain diameter was invariable. Under the same water stress and different soil grain diameter the expression of CesA gene was higher in the situation of 60%A＋40%B and 40%A＋60%B. when the water was normal, there was not obvious distinction.4. Analysised the regulations of the content of ABA,IAA,GA in different soil grain diameter.5. Deduced the relation between endogenesis hormone and expression of CesA gene.