Tissue Culture Of Oxalis Triangularis Cv. Purpurea

This paper used leafstalks, leaves and squamae as explants to research the medium conditions for callus inductions and organogenesis of Oxalis triangularis cv.purpurea, and set up vitro cell regeneration and cell suspension culture.With the research into dynamics of cell suspension culture,to analyze the best conditions for vegetal diversifications of callus inductions suspension culture,then set up an quick multiplication with high reproductive rates and efficiency. All of these made a basis for large-scale culture cell embryo with the bioreactor configuration and provided theoretical rationale for large-scale industrialized production. The article confirmed the amount of chromosome and the karyotype formulae by analyzing chromosome karyotype of Oxalis triangularis cv.purpurea .The results showed as follows: When the leaves were used as explants, the optimal concentration for callus induction is MS + 6-BA 0.5mg/L + 2,4-D 2.5mg/L + NAA 0.2mg/ L + saccharos 4%. When the leafstalks were used as explants, the optimal concentration for callus induction is MS + 6-BA 0.5mg/L + 2,4-D 2.5mg/ L + NAA 0.2mg/ L + saccharos 4%. When the squamae were used as explants, the optimal concentration for callus induction is MS + 6-BA 0.5mg/L + 2,4-D 2.5mg/L + NAA 0.2mg/L + saccharos 4%. The optimal concentration for callus subculture induction is MS + 6-BA 0.5mg/L + 2,4-D 1.5mg/L + saccharos 4%. The results showed that the optimum callus inoculum size, rotation speed and cell subculture cycle were 1.5g/30ml(wet weight),100r/min and 5～6 days respectively for callus suspension of Oxalis triangularis. The different physiological phases of Oxalis triangularis cv. purpurea during suspension culture were as follows: Stagnation stage was 0～3d, the logarithm growth time was 4～8d and the next days were stable and death stage,the cell multiplication rate could reach to 205%,the growth curve presented "S" type.The transformation of substrate kinetics during suspension culture were as follows: saccharos was broke down to dextrose and fructose by cells during suspension culture, dextrose was used on prior status and the utilized of fructose was lag behind comparatively, the portion of sugar was decreased slowly during cells'growth. During the suspension culture, electric conductivity and pH also changed correspondingly. During the initial stages, the electric conductivity decreased with the growth of callus, but after 8 days, it increased a little; and to pH, it increased at first, after 3 days, it increased, but always below it's initial figure.The climax of karyokinesis is on 8:30～9:30 everyday, so, the best time for fetching material is 9:00, The chromosome quantity is 2n=28, and the karyotypie formula is 2n=28=2M + 9m + 3sm, belong to the type of 2A.