Effect Of VitaminB₁, VitaminB₁₂ And VitaminH On The Growth And Astaxanthin Content Of Haematococcus Pluvialis

Haematococcus pluvialis that belongs to Chlorophata, Chlorophyceae,Volvocales, Haematococcaceae is a single economic microalgae and accumulates remarkable amounts of natural astaxanthin, which is an excellent feed additive, food colorant and a potential medicine. In order to grope for effect of Vitamin-B₁, Vitamin-B₁₂ and Vitamin-H on the growth and astaxanthin content of Haematococcus pluvialis. The experiments of Adding Vitamin-B₁, Vitamin-B₁₂ and Vitamin-H are setup,and is conducted for adding respectively, adding two kinds together and adding three kinds together. The experiments is up to seven groups. Cell density, biomass, chlorophyll-a content were measured. The results indicated that: The growth of H. pluvialis was improved significantly by adding of Vitamin-B₁, Vitamin-B₁₂ and Vitamin-H respectively, together two kinds or together three kinds. The cell density, biomass, chlorophyll-a content would obviously enhance with the increase of concentration of every group(except for the high concentration of every group). The growth of H. pluvialis in the Vitamin-B₁₂ group was better than other groups, and under the 50μg·L^-1 concentration of Vitamin-B₁₂, cell density, biomass, chlorophyll-a content were highest in all treatments and enhanced with 29.5%, 30.0%, 28.0% higher than the control. Astaxanthin content of H. pluvialis was accelerated significantly by adding of Vitamin-B₁, Vitamin-B₁₂ and Vitamin-H. when adding concentration of Vitamin-B₁₂ was 50μg·L^-1 astaxanthin content was highest(28.35μg·mL^-1), enhanced with 28.31 % than the control. Cell density and astaxanthin content were corresponding relation, properly adding Vitamin not only accelerated Cell density significantly but also enhance astaxanthin content. On the mass culture of motile cells of Haematococcus pluvialis properly adding Vitamin-B₁₂ was effective for increase cells density, biomass, chlorophyll-a and astaxanthin content.