Cloning Of Xeroderma Pigmentosum Complementation Group D (XPD) Gene And Construction Of Mammalian Expression Recombinant PEGFP-N2/XPD

Object XPD (Xeroderma pigmentosum group D)gene, also named ERCC2(X —ray repair cross-complementing group 2> gene, located in 19ql3. 2-3, including 23 exons. XPD protein, which is composed by 761 amino acids, with the 5' -3' direction helicase activity, is the second largest subunit of TFIIH. The transcription/DNA repair factor TFIIH is a complex enzyme made up of a total of nine subunits. Five of them (XPB, p62, p52, p44 and p34) form a tight core subcomplex. XPD is less tightly associated with the core and mediates the binding of the CAK subcomplex, which contains the remaining three subunits, cyclinH, cdk7, and MAT1. XPD presented as a bridge in TFIIH complex, its N-terminal part mainly binds the coiled-coil domain of MAT1 subunit of CAK subcomplex whereas its C terminus is integrated via holo-TFIIH and are stabilized by MAT1 via its hydrophobic domain. In nucleotide excision repair (NER), the 5' -3' direction helicase activity of XPD unwinds the DNA around the damaged site to permit damage-specific nucleases to cleave the DNA on either side of the damage, so that the damaged section can be removed. In the transcription, XPD needs to present to maintain the stability of the complex for transcription initiation and...