Analysis Of Genetic Diversity In Castanopsis Hystrix A.DC.

Castanopsis hystrix A.DC. is a kind of evergreen arbors having many eximious characters such as : growing fast,excellent texture,abroad acclimatization.In this study,inter-simple sequence repeat(ISSR),peroxidese isozymes,esterase isoenzymes,analysis of rbcL gene sequences were evaluated for its potential use in the genetic variability of 10 cultivars of Castanopsis hystrix A.DC.,and then combined with other physiological analysis, we can do a all-sides research about these 10 cultivars of Castanopsis hystrix A.DC. The mainly important experiment results were as follows:1. ISSR analysisStatis software was used to calculate the Nei's genetic distance and a dendrogram was constructed based on UPGMA cluster analysis. These 10 cultivars surveyed were classified into 4 major groups.After many tries, we find the optimum programming of ISSR-PCR ,and it is: template 60 ng,1 U Taq,1.5 mmol/LMg2+,dNTP 0.25 mmol/L, primers 0.5μmol/L,2%dimethyl formamide dimethyl acetal in 20μL reaction system.The proceeding of ISSR-PCR is:94℃5 min, 94℃30 s,52～55℃45 s,72℃2 min,40 cycles,72℃7 min.2. Isozymes analysisTwo isozymes(peroxidese isozyme,esterase isoenzyme) of 10 cultivars were studied by means of polyacrylamid gel electhophoresis.The results showed that several basic isozyme bands were observed in all cultivars and they have their own characteristic bands which are different from each other. Analysis of peroxidese isoenzyme showes that these three bands distribute in two zones(A,B),Pubei has one special band in C-zone. Analysis of esterase isoenzyme showes that these seven bands...