Study On Electrochemical Biosensor Of Glutamate Receptor Based On Biomembrane

As the living model membrane, biomembrane is very similar to the natural biomembrane with many characteristics such as fluidity and self-sealing. It includes self-assembly monolayer membrane, Langmuir-Blodgett membrane, bilayer membrane and multilayer membranes, and provides an environment for the interaction between the receptor and ligand, and keeping their activities at the same time. The biomembrane can obviously decrease the background noise and prevent other hydrophilic electroactive substances in the substrate from reaching the sensitive membrane and resulting an interference for analysis, therefore effectively improve the selectivity. As these characteristics are very fit for the construction of biosensor, much attention has been paid on BLM by many acientists.In this work, two kinds of biomembrane suitable for the construction of receptor sensor were prepared and characterized by different electrochemical methods such as EIS, CV, and et al. Based on which, an electrochemical biosensor of glutamate receptor was developed by incorporating the Glu receptor into biomembrane. It has been proved to provide useful information for the interaction of glutamate analyte and biomembrane with Glu receptor.The electrochemical response to the interaction would be a foundation for the detection of glutamate.The main research contents of this paper are as follows:1. By the survery of past literatures, the composition and application of biomembrane were simply introduced. Basic concept of glutamate and glutamate receptor, and the application of electrochemical impedance spectroscopy (EIS) measurements were also described elaborately.2. The Au/mercaptan monolayers were prepared by constant-potential assembly and self assembly in neutral environment. The preparation conditions were optimized, and the properties of SAMs were also characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements. Research result indicates that the monolayer of thiols with fewer defects can be prepared by both two method.3. The hybrid bilayer membrane (HBM) were prepared by the painted method and the paint-freeze method in the neutral environment, and the preparation...